Oh yes. There are actually certain places that I couldn't work because I don't wear makeup, besides even just theater/actress or stripper. My sister was expected to wear makeup working at Macy's behind a counter, and it was just the accessories counter, not actually the makeup counter. I believe we have gotten a lot better about dropping that expectation, but it still does exist. Even going out when I was younger, the expectation was obvious, although it seems to come from other women (from my personal observations) more than men.
Now, in my family, wearing makeup is actually odd, especially since my mother didn't wear it, so two of my sisters wear it pretty much all the time, whenever they leave the house, and the other sister and I do not wear it. But my MIL has a hard time leaving the house without putting on some makeup (although even she has said that is changing).